OPTIMIZATION OF CELLULASE PRODUCTION FROM Apergillus flavipes BY SUBMERGED AND SOLID STATE FERMENTATION

Cellulase are important class of enzymes that has potential applications in textile, biofuel, food and feed industry. Fungi such as Aspergillus, Trichoderma reliable sources cellulase production. Optimisation multiple parameters is required for efficient production high titers both SmF SSF. Carbon lactose, maltose, cellulose, nitrogen including ammonium dihydrogen phosphate beef extract enhance activities. Thiamine, Nicotinic acid all metal chlorides except CuCl2 enhanced Based on the one factor at a time model, optimum medium was designed which produced 0.767 FPU/ml by SmF. Maximum CMCase, FPase activity protein were pH 4.5 150 rpm with working volume ratio (wvr) 0.2. The 4.6 fold higher than un-optimized media. SSF an ideal fermentation method fungi. pNPG activities recorded 50% moisture level, 5.0 below 40 °C. Wheat bran acted best carbon source FPase, CMCase (5.6 IU/g, 17 IU/g 14 source) A. flavipes thus acts perfect fungal